paraffiinilised

Paraffinization, also known as paraffin wax embedding, is a laboratory technique used in histology and other scientific fields to prepare tissue samples for study. The process involves soaking the tissue sample in a paraffin wax solution, which replaces the water in the tissue, allowing for easier handling and sectioning of the sample.

The paraffinization process typically begins with the dehydration of the tissue sample using a series of ethanol or methanol washes. This step helps to remove water from the tissue, making it more receptive to the paraffin wax. The tissue sample is then submerged in a paraffin wax bath, where it is heated and allowed to soak for several hours or overnight. The paraffin wax dissolves the cell membranes and other cellular structures, allowing the tissue to become uniformly impregnated with the wax.

Once the paraffinization process is complete, the tissue sample can be embedded in a paraffin block, which is then cut into sections using a microtome. These sections are typically 4-6 micrometers thick and can be stained using various histological techniques to highlight cellular structures and other features of interest. Paraffinization is an essential step in many histological and cytological procedures, enabling researchers to study the morphology and ultrastructure of tissue samples in detail.

The paraffinization process has undergone several modifications and improvements over the years, including the use of automated embedding systems and specialized paraffin waxes. Despite these advances, the basic principles of paraffinization remain the same, and the technique continues to be widely used in scientific research and diagnostic medicine.