m7G527

m7G527 is a designation used in RNA biology to refer to a site in which a guanosine residue at position 527 bears a 7-methylguanosine (m7G) modification. The 527 refers to a coordinate within a reference RNA sequence or genome assembly, and the specific RNA bearing the modification may vary among studies or species. m7G denotes a methyl group added at the N7 position of guanine, a modification most widely known in the 5' cap structure of eukaryotic mRNAs but also found as internal m7G sites in various RNAs.

In practice, m7G527 has been reported in datasets mapping RNA modifications where the modified residue lies at or near position 527 of a defined transcript. The functional interpretation depends on the RNA context; in cap structures, m7G is essential for RNA stability and translation initiation, whereas internal m7G modifications can influence folding, processing, or ribosome association.

Biochemically, internal m7G modifications are catalyzed by RNA methyltransferases such as METTL1-WDR4 for internal m7G46 in some organisms, while cap-related m7G formation involves cap-specific enzymes like RNGTT and RNMT-RAM; the exact enzymes affecting m7G527 are subject to organism- and transcript-specific differences.

Detection typically relies on mass spectrometry, specialized RNA sequencing approaches, or primer-extension mapping. Overall, m7G527 is a site-specific example illustrating the broader theme of RNA methylation in gene expression regulation.

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