kolorimetery

Kolorimetery is a branch of analytical chemistry that deals with the quantitative determination of the concentration of a substance in a solution by measuring the amount of light absorbed or transmitted by the solution. The principle behind kolorimetery is based on the Beer-Lambert law, which states that the absorbance of a solution is directly proportional to the concentration of the absorbing species and the path length of the light through the solution.

In kolorimetery, a colorimetric reagent is added to the solution, which reacts with the analyte to form a colored complex. The intensity of the color produced is proportional to the concentration of the analyte. The absorbance of the solution is then measured using a spectrophotometer, which detects the amount of light absorbed or transmitted by the solution at a specific wavelength.

Kolorimetery is widely used in various fields, including clinical chemistry, environmental monitoring, and industrial quality control. It is a simple, rapid, and cost-effective method for quantitative analysis of a wide range of analytes, including metals, organic compounds, and biological molecules. However, the accuracy and reliability of kolorimetery depend on the proper selection of the colorimetric reagent and the conditions of the measurement.