hematoksiliinieosiinivärjäys
Hematoksiliinieosiinivärjäys, often abbreviated as H&E stain, is the most widely used staining method in histology. It is a cornerstone technique for examining tissue samples under a microscope. The process involves two main dyes: hematoxylin and eosin. Hematoxylin is a basic dye that stains acidic structures, such as the nucleus of a cell, a blue-purple color. Eosin is an acidic dye that stains basic structures, like the cytoplasm and extracellular matrix, in shades of pink or red. The differential staining allows pathologists and researchers to visualize cellular and tissue morphology, identify abnormal cells or tissues, and diagnose diseases. This technique is crucial in various fields, including medical diagnostics, research, and education, providing essential visual information about tissue composition and organization. The precise intensity and hue of the stains can vary depending on the specific protocol and reagents used, but the fundamental principle of nuclear and cytoplasmic differentiation remains consistent. H&E staining is relatively inexpensive, robust, and produces consistently interpretable results, contributing to its widespread adoption in laboratories worldwide.