calibrationskurvor

A calibration curve, also known as a standard curve, is a graph used in analytical chemistry to determine the concentration of a substance in an unknown sample. It is created by measuring the response of a detection instrument to a series of known concentrations of the substance, called standards. The instrument's response, such as absorbance, fluorescence, or peak area, is plotted against the corresponding known concentrations.

Once the calibration curve is established, the instrument's response to the unknown sample is measured. This response is then located on the y-axis of the calibration curve, and the corresponding concentration is read off the x-axis. This allows for the quantitative determination of the analyte in the unknown sample.

The accuracy of a calibration curve depends on several factors, including the quality of the standards, the precision of the instrument, and the range of concentrations covered. Ideally, the unknown sample's response should fall within the linear range of the calibration curve. Linear regression is often used to fit a line to the data points, providing an equation that relates the instrument's response to the concentration. The R-squared value from the regression indicates how well the data fits the line.