Subdiffractieresolutie

Subdiffractieresolutie, also known as superresolution, refers to a set of techniques that enable the resolution of an image to a finer scale than is possible with conventional diffraction-limited imaging. This is achieved by bypassing the traditional limitations imposed by the diffraction of light, which typically restricts the resolution to about half the wavelength of the light used.

One of the most well-known superresolution techniques is Stimulated Emission Depletion (STED) microscopy, developed by Stefan Hell and his team. STED uses a doughnut-shaped beam of light to selectively switch off fluorescent molecules in the sample, allowing the remaining molecules to emit light and form a high-resolution image. Another notable method is Structured Illumination Microscopy (SIM), which uses patterned illumination to create overlapping images that can be computationally combined to achieve superresolution.

Superresolution techniques have revolutionized fields such as biology and materials science, enabling researchers to study structures and processes at the nanoscale. They have applications in medical imaging, where they can improve the resolution of biological tissues, and in nanotechnology, where they aid in the characterization of materials and devices.

Despite their advantages, superresolution techniques also present challenges, including the need for specialized equipment and the potential for photobleaching and phototoxicity in biological samples. Ongoing research aims to address these issues and further enhance the capabilities of superresolution imaging.