STRTseq
STRTseq, also known as Single-cell Tagged Reverse Transcription sequencing, is a high-throughput method for profiling the transcriptome of individual cells. It allows for the simultaneous measurement of gene expression and other cellular features. The core principle of STRTseq involves capturing messenger RNA (mRNA) molecules from single cells and tagging them with a unique molecular identifier (UMI) and a cell barcode during the reverse transcription process. This tagging is crucial for distinguishing between multiple copies of the same mRNA molecule and for assigning each transcript to its originating cell.
The STRTseq protocol typically begins with cell lysis and the addition of reagents that facilitate the capture