RNAsekvenssiä

RNAsekvenssiä, often referred to as RNA sequencing or RNA-Seq, is a powerful molecular biology technique used to determine the complete RNA transcriptome of a cell or organism. It involves converting RNA molecules into complementary DNA (cDNA) fragments, which are then sequenced using next-generation sequencing (NGS) technologies. The resulting sequence reads are mapped back to a reference genome or transcriptome to identify and quantify the different RNA species present.

The primary application of RNA-Seq is to study gene expression. By measuring the abundance of RNA transcripts, researchers can understand which genes are active and to what extent under specific conditions. This allows for the investigation of various biological processes, such as cellular differentiation, disease development, and responses to environmental stimuli. Beyond gene expression, RNA-Seq can also detect novel transcripts, splice variants, and RNA modifications. It has become an indispensable tool in transcriptomics research, providing a comprehensive snapshot of cellular RNA content.