Nanoporesekvensointijärjestelmillä

Nanopore sequencing is a method of DNA sequencing that involves passing a single DNA molecule through a protein nanopore. As the DNA strand moves through the pore, it disrupts an electric current passing through it. This disruption creates a unique electrical signal that is detected and recorded. The sequence of these signals can then be translated into the corresponding DNA sequence. The key advantage of nanopore sequencing is its ability to perform long-read sequencing, meaning it can read much longer stretches of DNA in a single pass compared to some other sequencing technologies. This is particularly useful for assembling genomes, identifying structural variations, and analyzing repetitive regions. Another benefit is its real-time data output, allowing researchers to analyze results as they are generated. The technology can also be performed on portable devices, making it suitable for fieldwork and rapid diagnostics. Challenges include achieving high accuracy rates, although ongoing technological advancements are continuously improving this aspect. Nanopore sequencing has applications in a wide range of fields, including genomics, transcriptomics, epigenetics, and infectious disease surveillance.