NGSMethoden

NGSMethoden refers to the various techniques and protocols used in Next-Generation Sequencing (NGS), also known as high-throughput sequencing. These methods have revolutionized genomics by enabling the rapid and cost-effective sequencing of DNA and RNA at an unprecedented scale. The core principle of most NGS methods involves the preparation of DNA or RNA libraries, followed by massively parallel sequencing of millions of DNA fragments simultaneously.

Key steps in NGSMethoden typically include sample preparation, library construction, sequencing, and data analysis. Sample preparation involves isolating DNA or RNA from a biological source. Library construction then fragments the nucleic acid and ligates adapter sequences to the ends. These adapter sequences are crucial for binding to the sequencing platform and for amplification.

The actual sequencing process varies depending on the specific NGS platform used, but common approaches include sequencing-by-synthesis, where fluorescently labeled nucleotides are incorporated and detected as DNA is synthesized, and sequencing-by-ligation, which uses fluorescently labeled oligonucleotide probes. After sequencing, the raw data is processed through sophisticated bioinformatics pipelines to assemble the sequences, identify variations, and perform downstream analyses such as gene expression profiling or variant calling. Different NGSMethoden are optimized for various applications, from whole-genome sequencing to targeted sequencing of specific genes or exons.