G4Base

G4Base is a public, curated database that catalogs G-quadruplex forming sequences and related annotations across genomes. It serves researchers studying G4 DNA structures and their roles in transcription, replication, and genome stability. The database integrates computational predictions with experimental evidence to provide a comprehensive resource for identifying potential G-quadruplex motifs and their genomic context.

G4Base includes predicted G4 motifs identified by multiple algorithms, experimentally validated G4 sites from high-throughput studies, and associated metadata such as organism, chromosome, coordinates, strand, and flanking sequence context. Each entry carries supporting evidence, a confidence score, and links to primary literature or data sources. The platform also records data provenance and version history to support reproducibility.

Users can search by sequence, gene, genomic location, or score thresholds, and browse through a lightweight genome view. Advanced filters enable organism, experimental method, or motif characteristics. Bulk downloads are available in multiple formats (FASTA, BED, CSV), and a RESTful API provides programmatic access for integration into analysis pipelines.

G4Base relies on a combination of automated pipelines and manual curation. Submissions from the community are reviewed before integration, and new releases incorporate updated annotations and corrections. The project maintains documentation, usage guidelines, and citation information for data reuse.

G4Base is freely accessible to the research community. Data are distributed under an open license, with guidelines for attribution and reuse. The resource aims to be interoperable with related genomics and structural biology resources.