Agarosegeeli
Agarose gel, also known as agarosegeeli in some languages, is a porous matrix used in gel electrophoresis to separate nucleic acids by size. It is prepared by dissolving agarose powder in a suitable buffer, heating to dissolve, and then casting the solution in a mold with a comb to form wells. After it solidifies, a running buffer is added and samples are loaded for electrophoresis.
Agarose is a natural polysaccharide derived from red seaweed. The pore size of the gel depends on
Common applications include sizing and analyzing PCR products, checking restriction enzyme digests, verifying plasmid constructs, and
Buffers such as TAE (Tris-acetate-EDTA) and TBE (Tris-borate-EDTA) are used with agarose gels. DNA is typically