cyanmethemoglobinmetoden

The cyanmethemoglobin method is a laboratory technique used to determine the concentration of hemoglobin in a blood sample. This method relies on the conversion of hemoglobin to cyanmethemoglobin, a stable colored compound, which can then be measured spectrophotometrically.

To perform the assay, a small volume of blood is mixed with a solution containing potassium ferricyanide and potassium cyanide. The potassium ferricyanide oxidizes the ferrous iron (Fe2+) in hemoglobin to ferric iron (Fe3+), forming methemoglobin. Subsequently, the potassium cyanide reacts with the methemoglobin to form cyanmethemoglobin. This cyanmethemoglobin molecule has a characteristic absorption spectrum with a peak absorbance at approximately 540 nanometers.

The intensity of the color produced, which is directly proportional to the hemoglobin concentration, is then measured using a spectrophotometer. A calibration curve, prepared using solutions of known hemoglobin concentrations, is used to determine the unknown hemoglobin concentration in the sample. The cyanmethemoglobin method is widely used due to its accuracy, reliability, and relative simplicity. It is considered a standard method for hemoglobin determination in clinical laboratories and research settings. The procedure is straightforward and can be automated for high-throughput analysis. However, it's important to note that certain interfering substances can affect the accuracy of the results, and proper sample handling and reagent quality are crucial for reliable measurements.