UVspektrometrisessä

UVspektrometrisessä is a term related to ultraviolet (UV) spectrophotometry. This analytical technique measures the absorption of ultraviolet light by a sample. When UV light passes through a substance, molecules within that substance can absorb specific wavelengths of light. The amount of light absorbed is directly proportional to the concentration of the absorbing substance.

UV spectrophotometry is a widely used method in chemistry, biochemistry, and pharmaceuticals. It is employed for identifying and quantifying chemical compounds that possess chromophores, which are molecular fragments capable of absorbing UV or visible light. Common applications include determining the concentration of proteins, nucleic acids, and various organic molecules. It is also used in quality control, drug analysis, and environmental monitoring. The instrument used, a UV spectrophotometer, typically consists of a light source (often a deuterium lamp or a tungsten lamp), a monochromator to select specific wavelengths, a sample holder, and a detector to measure the transmitted light intensity. The results are often presented as a spectrum showing absorbance versus wavelength.