UVVisiblespektreissä

UVVisible spectroscopy is a technique used to measure the absorption and transmission of light in the ultraviolet (UV) and visible portions of the electromagnetic spectrum. When a substance is exposed to UV or visible light, its molecules can absorb photons of specific wavelengths, causing electrons to transition to higher energy levels. The amount of light absorbed at each wavelength is then measured, creating a spectrum. This spectrum provides information about the electronic structure of the molecule. Different chemical compounds will absorb light at different wavelengths due to their unique molecular arrangements and the presence of chromophores, which are groups of atoms within a molecule responsible for its color and UV-Vis absorption properties. The resulting spectrum typically plots absorbance or transmittance against wavelength. UVVisible spectroscopy is widely used in various fields, including chemistry, biology, and materials science, for qualitative analysis (identifying substances) and quantitative analysis (determining the concentration of a substance). It can be used to study reaction kinetics, analyze the purity of samples, and characterize the properties of materials. The principle relies on Beer-Lambert Law, which states that the absorbance of a solution is directly proportional to the concentration of the absorbing species and the path length of the light through the solution.