UVVISspektrofotomeeter

A UVVIS spectrophotometer is an analytical instrument used to measure the amount of light absorbed or transmitted by a sample at specific wavelengths within the ultraviolet (UV) and visible (VIS) portions of the electromagnetic spectrum. These wavelengths typically range from 200 to 800 nanometers. The core principle of operation involves a light source that emits radiation across this spectrum. This radiation then passes through a monochromator, which isolates a narrow band of wavelengths. The monochromatic light then interacts with the sample, which is usually held in a cuvette. The detector measures the intensity of light that has passed through the sample. By comparing the intensity of the incident light to the intensity of the transmitted light, the spectrophotometer can determine the absorbance or transmittance of the sample at that particular wavelength. UVVIS spectrophotometry is a widely used technique in various scientific fields, including chemistry, biology, and environmental science, for quantitative analysis of substances, identification of unknown compounds, and kinetic studies of chemical reactions. It relies on the Beer-Lambert Law, which states that the absorbance of a solution is directly proportional to the concentration of the analyte and the path length of the light beam through the solution.