SequencingbySynthesisTechnologie

Sequencing by Synthesis (SBS) is a method used in DNA sequencing, which is the process of determining the precise order of nucleotides within a DNA molecule. This technique is particularly useful for sequencing short DNA fragments, such as those generated by next-generation sequencing platforms. In SBS, the DNA fragment is first attached to a solid support, typically a bead or a surface. The nucleotides are then added one by one, and each addition is detected by a fluorescent signal. The sequence is determined by the order in which the nucleotides are added. SBS is a rapid and accurate method for sequencing DNA, and it is widely used in research and clinical settings. However, it is limited by the length of the DNA fragments that can be sequenced, and it requires specialized equipment and reagents.