MLCKi

MLCKi, also known as MLC-1, is a myosin light chain kinase. It is an enzyme that plays a crucial role in regulating the interaction between actin and myosin, fundamental components of muscle contraction and various cellular processes. This kinase is a member of the calcium/calmodulin-dependent protein kinase (CaMK) family, meaning its activity is modulated by calcium ions and the calcium-binding protein calmodulin.

The primary function of MLCKi is to phosphorylate the regulatory light chain of myosin II. This phosphorylation event alters the conformation of myosin II, enhancing its ATPase activity and its ability to bind to actin filaments. Consequently, this leads to increased force generation in muscle cells and contributes to the contraction of smooth muscle. Beyond muscle, MLCKi is also involved in non-muscle cellular functions such as cell adhesion, cell migration, and cytokinesis. Its presence and activity are important for maintaining cellular structure and facilitating cellular movement. Dysregulation of MLCKi has been implicated in various pathological conditions, including hypertension, asthma, and certain types of cancer, highlighting its significance in both physiological and disease states.