spectrofmeters

Spectrophotometers are analytical instruments used to measure the amount of light absorbed or transmitted by a sample at specific wavelengths. This measurement is based on the principle that different substances absorb and transmit light differently depending on their chemical composition and concentration. The instrument works by passing a beam of light through a sample and then measuring the intensity of the light that emerges. The difference between the incident light intensity and the transmitted light intensity is used to determine the absorbance or transmittance of the sample. Spectrophotometers are widely used in various fields, including chemistry, biology, environmental science, and medicine, for qualitative and quantitative analysis of substances. They are essential tools for identifying unknown compounds, determining the concentration of known substances, and monitoring chemical reactions. The accuracy and precision of spectrophotometers make them indispensable for research, quality control, and diagnostic applications. Different types of spectrophotometers exist, including UV-Vis, infrared, and atomic absorption spectrophotometers, each designed for specific applications and wavelength ranges.