Spektrofotometrisiä

Spektrofotometrisiä refers to the measurement of the intensity of light as a function of wavelength. It is a technique used in chemistry and physics to determine the amount of a chemical substance present in a sample by measuring the amount of light it absorbs or transmits. A spectrophotometer is the instrument used for this purpose. It works by passing a beam of light through a sample and then measuring how much of that light passes through to a detector. The amount of light absorbed by the sample is related to the concentration of the substance present. Different substances absorb light at different wavelengths, creating a unique absorption spectrum that can be used to identify the substance. This technique is widely used for quantitative analysis, such as determining the concentration of solutions, identifying unknown compounds, and studying chemical reactions. The Beer-Lambert law is a fundamental principle that underpins many spectrophotometric analyses, stating that the absorbance of a solution is directly proportional to the concentration of the absorbing species and the path length the light travels through the solution. Variations of spectrophotometry include UV-Vis spectrophotometry, which measures light in the ultraviolet and visible regions of the electromagnetic spectrum, and infrared spectrophotometry, which measures in the infrared region.